edu磨課師+ 分子生物學 講師:李柏蒼 國立臺灣海洋大學 水產養殖學 系副教授 影音教學 英文發音 英文版(DVD版)
【課程簡介】
開課學校/機構:國立臺灣海洋大學
課程發展年度:2023
課程類別:自然科學_生物學
※ 此門課程為全英語課程 ※
Aquatic pathogen detection is essential for disease prevention and curation during culture periods, and molecular biology techniques such as polymerase chain reaction have become a well-established, accurate and effective way for pathogen detection that requires a much shorter time to obtain correct results compared to traditional microbiological methods. These techniques are very powerful in the field of not only aquaculture, but also other animal agriculture industries, and even in the hospital for human disease. Therefore, this course provides a basic concept of how to perform nucleic acid extraction and a few PCR-related techniques for aquatic pathogen detection.
【先備能力】
※ 此門課程為全英語課程 ※
This course is for any student, biologist, or researcher who would like to learn the basics of tissue collection, nucleic acid extraction and pathogen detection in white shrimp. It is designed for learners with no experience in programming whatsoever.
【學習目標】
※ 此門課程為全英語課程 ※
- Anatomy of white shrimp
- Knowledge of chromosomal DNA extraction from white shrimp tissues
- Knowledge and steps of RNA extraction from white shrimp tissues Procedures of RNA extraction and cDNA synthesis
- Knowledge and steps for standard polymerase chain reaction (PCR)
- Knowledge and steps for real-time quantitative polymerase chain reaction (qRT-PCR)
【授課教師】
李柏蒼
任職單位
國立臺灣海洋大學 水產養殖學 系副教授
簡述
學歷：英國亞伯丁大學博士 (Aberdeen University, U.K.)
研究領域：細菌性魚病學、免疫學、分子生物學、水產養殖學 (Bacterial Fish Pathology, Immunology, Molecular Biology, Aquaculture)
001_1-1-Introduction-to-Aquaculture-and-the-Importance-of-Pathogen-Detection.mp4
002_1-2-Methods-for-identifying-aquaculture-diseases.mp4
003_1-3-_-Shrimp-dissection-and-genomic-DNA-extraction.mp4
004_1-4-_-PCR-procedures.mp4
005_2-1_-How-to-isolate-RNA-from-the-sample--high-quality-RNA-extraction..mp4
006_2-2_-Step-to-optimal-cDNA-synthesis-part-1_-Sample-preparation.mp4
007_2-3_-Step-to-optimal-cDNA-synthesis-part-2_-Primer-selection-and-reaction-mix.mp4
008_2-4_-Perform-cDNA-synthesis-and-cDNA-amplification-in-PCR.mp4
009_-3-1-_-Preparation-of--PCR.mp4
010_3-2-_-How-does-PCR-work-.mp4
011_3-3-_-What-is-quantitative-real-time-PCR-(qPCR)--Ct-value-and-PCR-efficiency.mp4
012_-3-4-_-Absolute-quantification-and-Relative-quantification-(Livak-method).mp4
013_3-5-_-Relative-quantification-(Pfaffl-method).mp4
014_3-6_-Real-time-PCR-primer-design_-the-primers-in-different-situations.mp4
015_3-7--_-Real-time-PCR-primer-design_-step-of-primer-design-application.mp4
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